Journal: BMC Research Notes

Article Title: Mouse strain specific gene expression differences for illumina microarray expression profiling in embryos

doi: 10.1186/1756-0500-5-232

Figure Lengend Snippet: List of the top 50 targets ranked by total fold change following a strain by strain comparison after microarray based differential expression analysis for the following three data sets

Article Snippet: Since the results of microarray gene expression profiling can be impacted on by variations in the strain of mouse used, we aimed to provide a resource reference list of probes contributing to strain differences or “noise” when subjecting non-isogenic tissue from any of the frequently used inbred strains in mouse gene targeting or even an outbred strain, to microarray based differential gene expression profiling using the Illumina Mouse WG6 v2.0 microarray chip.

Techniques: Microarray, Expressing

Journal: BMC Research Notes

Article Title: Mouse strain specific gene expression differences for illumina microarray expression profiling in embryos

doi: 10.1186/1756-0500-5-232

Figure Lengend Snippet: Mouse strain gene ontology relationships for 11 strains based on microarray based differential gene expression analysis of eviscerated embryos at E12.5. For simplicity the strain names have been abbreviated in this tree view figure. The full strain name is given in brackets preceded by the abbreviation as following: 129 [129 S2/SvHsd]; FVBN [FVB/NHan TM Hsd]; C3H [C3H/HeNHs]; CBA [CBA/JHsd]; BalbC [BALB/cOlaHsd]; SCID [C.B-17/IcrHan TM Hsd- Prkdc scid ]; B6 [C57BL/6J]; Col2aCre [B6; SJL-Tg(Col2a1-cre)1Bhr/J]; ROSAFlpe [129 S4/SvJaeSor- Gt(Rosa)26Sor tm1(FLP1)Dym /J]; ZP3Cre [C57BL/6-Tg(Zp3-cre)93Knw/J] and CD1 [Hsd:ICR(CD-1)®)]. The numbers 1 to 4 for each group refer to the biological replicate.

Article Snippet: Since the results of microarray gene expression profiling can be impacted on by variations in the strain of mouse used, we aimed to provide a resource reference list of probes contributing to strain differences or “noise” when subjecting non-isogenic tissue from any of the frequently used inbred strains in mouse gene targeting or even an outbred strain, to microarray based differential gene expression profiling using the Illumina Mouse WG6 v2.0 microarray chip.

Techniques: Microarray, Expressing